{"id":88,"date":"2026-02-22T23:05:48","date_gmt":"2026-02-22T23:05:48","guid":{"rendered":"https:\/\/legacyantibodies.com\/?page_id=88"},"modified":"2026-03-19T11:14:22","modified_gmt":"2026-03-19T11:14:22","slug":"service-two","status":"publish","type":"page","link":"https:\/\/legacyantibodies.com\/en\/service-two\/","title":{"rendered":"Service Two"},"content":{"rendered":"<p>[et_pb_section fb_built=&#8221;1&#8243; admin_label=&#8221;Header&#8221; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; use_background_color_gradient=&#8221;on&#8221; background_color_gradient_stops=&#8221;rgba(31,35,44,0.7) 0%|gcid-9fa4a1b6-767d-41ca-984c-a6f10449c843 100%&#8221; background_color_gradient_overlays_image=&#8221;on&#8221; background_enable_image=&#8221;off&#8221; locked=&#8221;off&#8221; collapsed=&#8221;on&#8221; global_colors_info=&#8221;{%22gcid-9fa4a1b6-767d-41ca-984c-a6f10449c843%22:%91%22background_color_gradient_stops%22%93}&#8221;][et_pb_row _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;251b68b6-4e76-4c9d-b365-27e0cf9d8351&#8243; header_4_text_color=&#8221;gcid-2f3f3e09-0a26-4ea7-8906-5e1128945e3f&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;||10px||false|false&#8221; global_colors_info=&#8221;{%22gcid-2f3f3e09-0a26-4ea7-8906-5e1128945e3f%22:%91%22header_4_text_color%22%93}&#8221;]<\/p>\n<h4>Services<\/h4>\n<p>[\/et_pb_text][et_pb_image src=&#8221;https:\/\/legacyantibodies.com\/wp-content\/uploads\/2026\/02\/attorney-1.png&#8221; title_text=&#8221;attorney-1&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; max_width=&#8221;130px&#8221; module_alignment=&#8221;center&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_image][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;185dad7e-6b26-45fe-8ef2-4418c8ac3c7d&#8221; header_text_color=&#8221;#C6C6C6&#8243; text_orientation=&#8221;center&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h1>Monoclonal Antibodies<\/h1>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; admin_label=&#8221;Service Info&#8221; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; background_color=&#8221;gcid-9fa4a1b6-767d-41ca-984c-a6f10449c843&#8243; collapsed=&#8221;on&#8221; global_colors_info=&#8221;{%22gcid-9fa4a1b6-767d-41ca-984c-a6f10449c843%22:%91%22background_color%22%93}&#8221;][et_pb_row custom_padding_last_edited=&#8221;on|desktop&#8221; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; custom_padding=&#8221;30px|60px|30px|60px|true|true&#8221; custom_padding_tablet=&#8221;|40px||40px|false|true&#8221; custom_padding_phone=&#8221;20px|20px|20px|20px|true|true&#8221; border_color_all=&#8221;#848484&#8243; border_width_left=&#8221;5px&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;69c3f2f1-c4fd-4cad-970d-6845b60d5e43&#8243; header_3_text_color=&#8221;#C6C6C6&#8243; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h3>A step up<\/h3>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;5e25d352-f972-494d-8a7e-59c933b838d0&#8243; text_text_color=&#8221;#FFFFFF&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>Monoclonals offer the potential for a number of improvements over polyclonals should the requirements demand.\u00a0 Whereas the latter are a collection of all the antibodies made as a response to an antigen, with the former you can continuously screen clones until finding the one that works best for you.\u00a0 This clone can then be expanded to produce the same antibody as long as it is required, a huge benefit over the potential lot-to-lot variability found in polyclonals.\u00a0<\/p>\n<p>Our monoclonal offerings consist of two possible avenues:<\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=&#8221;1_2,1_2&#8243; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_2&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;de40b6ec-3ad3-412d-95db-a4ab03220b73&#8243; text_text_color=&#8221;#ffffff&#8221; text_font_size=&#8221;16px&#8221; header_4_text_color=&#8221;#C6C6C6&#8243; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p><strong><span style=\"text-decoration: underline;\">Mouse Hybridoma<\/span><\/strong><\/p>\n<ul>\n<li><strong>Phase 1:<\/strong> Injection of mice, test bleed with ELISA<\/li>\n<li><strong>Phase 2:<\/strong> Splenectomy of selected animal, fusion of splenocytes with myeloma cell line, multi-stage screen to isolate positive clones<\/li>\n<li><strong>Phase 3:<\/strong> Subcloning of selected parentals to isolate monoclones with stable reactivity<\/li>\n<li><strong>Phase 4 (optional):<\/strong> Expansion and purification at desired scale<\/li>\n<li><strong>Phase 5 (optional):<\/strong> Sequencing of selected clones<\/li>\n<\/ul>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_2&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;5e25d352-f972-494d-8a7e-59c933b838d0&#8243; text_text_color=&#8221;#FFFFFF&#8221; text_line_height=&#8221;1.7em&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p><strong><span style=\"text-decoration: underline;\">Rabbit B-cell sorting<\/span><\/strong><\/p>\n<ul>\n<li><strong>Phase 1a:<\/strong> Essentially a typical Core Package polyclonal<\/li>\n<li><strong>Phase 1b:<\/strong> Additional confirmatory ELISA, options for splenectomy of selected animal or whole blood collection, isolation of splenocytes\/PBMCs<\/li>\n<li><strong>Phase 2:<\/strong> Single cell sorting to isolate positives<\/li>\n<li><strong>Phase 3:<\/strong> Variable region cloning, transient expression as LEM, ELISA<\/li>\n<li><strong>Phase 4 (optional):<\/strong> Sequencing of VH\/VL chains, stable expression, in mammalian cell line, small scale purification by protein A, ELISA and SDS-PAGE<\/li>\n<li><strong>Phase 5 (optional):<\/strong> Scale up to 1L production, purification by protein A, SDS-PAGE, final QC packet<\/li>\n<\/ul>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=&#8221;1_4,3_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;de40b6ec-3ad3-412d-95db-a4ab03220b73&#8243; header_4_text_color=&#8221;#C6C6C6&#8243; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h4>How to decide<\/h4>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;3_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_blurb title=&#8221;Mouse projects average 5-6 months while rabbit projects average 6-7 months&#8221; use_icon=&#8221;on&#8221; font_icon=&#8221;&#xe025;||divi||400&#8243; image_icon_background_color=&#8221;#848484&#8243; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;a73f4a29-f37a-476c-b96b-b361e6940cba&#8221; custom_margin=&#8221;||20px||false|false&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_blurb][et_pb_blurb title=&#8221;Average cost of rabbit project is roughly 2x that of mouse&#8221; use_icon=&#8221;on&#8221; font_icon=&#8221;&#xe0ed;||divi||400&#8243; image_icon_background_color=&#8221;#848484&#8243; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;a73f4a29-f37a-476c-b96b-b361e6940cba&#8221; custom_margin=&#8221;||20px||false|false&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_blurb][et_pb_blurb title=&#8221;Typical mouse project ends with cell line to be stored, while rabbit nets a purified recombinant with known sequences&#8221; use_icon=&#8221;on&#8221; font_icon=&#8221;&#xe0ff;||divi||400&#8243; image_icon_background_color=&#8221;#848484&#8243; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;a73f4a29-f37a-476c-b96b-b361e6940cba&#8221; custom_margin=&#8221;||20px||false|false&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_blurb][et_pb_blurb title=&#8221;Rabbits have a more robust immune response than mice, and a higher number of initial clones can be tested&#8221; use_icon=&#8221;on&#8221; font_icon=&#8221;&#xe106;||divi||400&#8243; image_icon_background_color=&#8221;#848484&#8243; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;a73f4a29-f37a-476c-b96b-b361e6940cba&#8221; custom_margin=&#8221;||20px||false|false&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_blurb][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=&#8221;1_4,3_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;de40b6ec-3ad3-412d-95db-a4ab03220b73&#8243; header_4_text_color=&#8221;#C6C6C6&#8243; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h4>Legacy can help<\/h4>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;3_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;5e25d352-f972-494d-8a7e-59c933b838d0&#8243; text_text_color=&#8221;#FFFFFF&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>Whatever your needs, preferences, or driving forces, we are happy to discuss your project and offer an appropriate solution to help lead you towards success.<\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; admin_label=&#8221;Footer&#8221; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; use_background_color_gradient=&#8221;on&#8221; background_color_gradient_stops=&#8221;#1e232b 0%|rgba(30,35,43,0.9) 100%&#8221; background_color_gradient_overlays_image=&#8221;on&#8221; background_enable_image=&#8221;off&#8221; saved_tabs=&#8221;all&#8221; locked=&#8221;off&#8221; collapsed=&#8221;on&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row column_structure=&#8221;1_3,1_3,1_3&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_3&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.5&#8243; _module_preset=&#8221;370b430b-b3b2-4e61-8111-d9ef05ce5ad6&#8243; header_4_text_color=&#8221;#C6C6C6&#8243; text_orientation=&#8221;center&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h4>Office Hours<\/h4>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;2023cf4b-5667-411c-ac82-879e66de99ac&#8221; text_text_color=&#8221;#FFFFFF&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;||10px||false|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>M &#8211; F 7:00 am to 4:00 pm (ET)<\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_3&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_image src=&#8221;https:\/\/legacyantibodies.com\/wp-content\/uploads\/2026\/03\/Logo-final.png&#8221; title_text=&#8221;Logo final&#8221; align=&#8221;center&#8221; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; max_height=&#8221;90px&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_image][\/et_pb_column][et_pb_column type=&#8221;1_3&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.5&#8243; _module_preset=&#8221;370b430b-b3b2-4e61-8111-d9ef05ce5ad6&#8243; header_4_text_color=&#8221;#C6C6C6&#8243; text_orientation=&#8221;center&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h4>Contact Info<\/h4>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;2023cf4b-5667-411c-ac82-879e66de99ac&#8221; text_text_color=&#8221;#FFFFFF&#8221; link_text_color=&#8221;#FFFFFF&#8221; text_orientation=&#8221;center&#8221; link_option_url=&#8221;https:\/\/maps.app.goo.gl\/CaoyZMXxzzU1jd8o6&#8243; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>2 Shaker Rd., Suite B203<\/p>\n<p>Shirley, MA 01464<\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;2023cf4b-5667-411c-ac82-879e66de99ac&#8221; text_text_color=&#8221;#FFFFFF&#8221; link_text_color=&#8221;#FFFFFF&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;||10px||false|false&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p><a href=\"tel:+15089227719\">+1 (508) 922-7719<\/a><\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;2023cf4b-5667-411c-ac82-879e66de99ac&#8221; text_text_color=&#8221;#FFFFFF&#8221; link_text_color=&#8221;#FFFFFF&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;||10px||false|false&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p><a href=\"mailto:scottedwinlewis4@gmail.com\">scott@legacyantibodies.com<\/a><\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; custom_padding=&#8221;0px||0px||true|&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_social_media_follow icon_color=&#8221;#000000&#8243; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;||||false|false&#8221; hover_enabled=&#8221;0&#8243; border_radii=&#8221;on|100px|100px|100px|100px&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221; sticky_enabled=&#8221;0&#8243;][et_pb_social_media_follow_network social_network=&#8221;linkedin&#8221; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; background_color=&#8221;#848484&#8243; background_enable_color=&#8221;on&#8221; custom_padding=&#8221;8px|8px|8px|8px|true|true&#8221; global_colors_info=&#8221;{}&#8221; follow_button=&#8221;off&#8221; url_new_window=&#8221;on&#8221; url=&#8221;https:\/\/www.linkedin.com\/company\/legacy-antibodies-llc&#8221; hover_enabled=&#8221;0&#8243; sticky_enabled=&#8221;0&#8243;]linkedin[\/et_pb_social_media_follow_network][\/et_pb_social_media_follow][et_pb_divider color=&#8221;#848484&#8243; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;||0px||false|false&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_divider][et_pb_text _builder_version=&#8221;4.27.5&#8243; _dynamic_attributes=&#8221;content&#8221; _module_preset=&#8221;84a07382-e7a7-4f9c-845a-4ad31f3c7af8&#8243; text_text_color=&#8221;#FFFFFF&#8221; text_orientation=&#8221;center&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoiY3VycmVudF9kYXRlIiwic2V0dGluZ3MiOnsiYmVmb3JlIjoiQ29weXJpZ2h0ICZjb3B5OyAiLCJhZnRlciI6IiBMZWdhY3kgQW50aWJvZGllcy4gQWxsIFJpZ2h0cyBSZXNlcnZlZC4iLCJkYXRlX2Zvcm1hdCI6ImN1c3RvbSIsImN1c3RvbV9kYXRlX2Zvcm1hdCI6IlkifX0=@[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>ServicesMonoclonal AntibodiesA step upMonoclonals offer the potential for a number of improvements over polyclonals should the requirements demand.\u00a0 Whereas the latter are a collection of all the antibodies made as a response to an antigen, with the former you can continuously screen clones until finding the one that works best for you.\u00a0 This clone can [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"<!-- 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