{"id":92,"date":"2026-02-22T23:06:24","date_gmt":"2026-02-22T23:06:24","guid":{"rendered":"https:\/\/legacyantibodies.com\/?page_id=92"},"modified":"2026-03-19T11:15:53","modified_gmt":"2026-03-19T11:15:53","slug":"service-four","status":"publish","type":"page","link":"https:\/\/legacyantibodies.com\/en\/service-four\/","title":{"rendered":"Service Four"},"content":{"rendered":"<p>[et_pb_section fb_built=&#8221;1&#8243; admin_label=&#8221;Header&#8221; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; use_background_color_gradient=&#8221;on&#8221; background_color_gradient_stops=&#8221;rgba(31,35,44,0.7) 0%|gcid-9fa4a1b6-767d-41ca-984c-a6f10449c843 100%&#8221; background_color_gradient_overlays_image=&#8221;on&#8221; background_enable_image=&#8221;off&#8221; locked=&#8221;off&#8221; collapsed=&#8221;on&#8221; global_colors_info=&#8221;{%22gcid-9fa4a1b6-767d-41ca-984c-a6f10449c843%22:%91%22background_color_gradient_stops%22%93}&#8221;][et_pb_row _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;9e37a409-6d37-4e97-a425-2b9a2fe75de5&#8243; header_4_text_color=&#8221;#C6C6C6&#8243; text_orientation=&#8221;center&#8221; custom_margin=&#8221;||10px||false|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h4>Services<\/h4>\n<p>[\/et_pb_text][et_pb_image src=&#8221;https:\/\/legacyantibodies.com\/wp-content\/uploads\/2026\/02\/attorney-1.png&#8221; title_text=&#8221;attorney-1&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; max_width=&#8221;130px&#8221; module_alignment=&#8221;center&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_image][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;986a248c-23cb-4cf2-88ec-85b68a6de11c&#8221; header_text_color=&#8221;#C6C6C6&#8243; text_orientation=&#8221;center&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h1>Proteomic Antibodies<\/h1>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; admin_label=&#8221;Service Info&#8221; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; background_color=&#8221;gcid-9fa4a1b6-767d-41ca-984c-a6f10449c843&#8243; collapsed=&#8221;on&#8221; global_colors_info=&#8221;{%22gcid-9fa4a1b6-767d-41ca-984c-a6f10449c843%22:%91%22background_color%22%93}&#8221;][et_pb_row custom_padding_last_edited=&#8221;on|desktop&#8221; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; custom_padding=&#8221;30px|60px|30px|60px|true|true&#8221; custom_padding_tablet=&#8221;|40px||40px|false|true&#8221; custom_padding_phone=&#8221;20px|20px|20px|20px|true|true&#8221; border_color_all=&#8221;#848484&#8243; border_width_left=&#8221;5px&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;c1930455-7ecc-4a4e-9385-94c793d63c18&#8243; header_3_text_color=&#8221;#C6C6C6&#8243; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h3>Anti-peptide antibodies for use in mass spec assay workflows<\/h3>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;26d90564-5812-48ea-af75-f2e9288726fd&#8221; text_text_color=&#8221;#FFFFFF&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>Antibodies to tryptic (or other enzyme derived) fragments present an excellent solution for highly accurate and quantifiable measurement of protein targets isolated from biological samples.\u00a0 We at Legacy have been at the forefront of method and protocol development for the creation of these reagents for the last 15 years, making thousands of critically useful tools for various applications.<\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=&#8221;1_4,3_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;73ca279a-c4bc-476b-9b19-0f06937dc911&#8243; header_4_text_color=&#8221;#C6C6C6&#8243; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h4>Our Methods<\/h4>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;3_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;26d90564-5812-48ea-af75-f2e9288726fd&#8221; text_text_color=&#8221;#FFFFFF&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>The process for this essentially follows our Core Polyclonal service, yet there are some key differences:<\/p>\n<ul>\n<li>The client typically provides the target sequence(s) based on internal MS results rather than have us perform antigen design.\u00a0 We are happy to make recommendations pertaining to antigenicity or sequence difficulty to help narrow down the scope as needed.<\/li>\n<li>Affinity purification includes a number of additional steps to ensure the resulting antibodies are &#8216;clean&#8217;, i.e., with very low background signal.<\/li>\n<li>A heavy isotope labeled version of the peptide is used for affinity purification, albeit one different from the antigen peptide or heavy standard typically used in the final assay.<\/li>\n<li>Custom heavy and light peptide standards can be produced and added to the order as needed.<\/li>\n<\/ul>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=&#8221;1_4,3_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;73ca279a-c4bc-476b-9b19-0f06937dc911&#8243; header_4_text_color=&#8221;#C6C6C6&#8243; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h4>Advanced Needs<\/h4>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;3_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;26d90564-5812-48ea-af75-f2e9288726fd&#8221; text_text_color=&#8221;#FFFFFF&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>Just as with any polyclonal project, it is possible to progress your project to a rabbit monoclonal if you need a more permanent form of the reagent to carry forward into higher scales or more secured production.\u00a0 It is beneficial &#8211; but not required &#8211; to know this at the onset of the project, as early prep towards this end can avoid potential delays later in the process.<\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; admin_label=&#8221;Footer&#8221; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; use_background_color_gradient=&#8221;on&#8221; background_color_gradient_stops=&#8221;#1e232b 0%|rgba(30,35,43,0.9) 100%&#8221; background_color_gradient_overlays_image=&#8221;on&#8221; background_enable_image=&#8221;off&#8221; saved_tabs=&#8221;all&#8221; locked=&#8221;off&#8221; collapsed=&#8221;on&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row column_structure=&#8221;1_3,1_3,1_3&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_3&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.5&#8243; _module_preset=&#8221;370b430b-b3b2-4e61-8111-d9ef05ce5ad6&#8243; header_4_text_color=&#8221;#C6C6C6&#8243; text_orientation=&#8221;center&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h4>Office Hours<\/h4>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;2023cf4b-5667-411c-ac82-879e66de99ac&#8221; text_text_color=&#8221;#FFFFFF&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;||10px||false|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>M &#8211; F 7:00 am to 4:00 pm (ET)<\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_3&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_image src=&#8221;https:\/\/legacyantibodies.com\/wp-content\/uploads\/2026\/03\/Logo-final.png&#8221; title_text=&#8221;Logo final&#8221; align=&#8221;center&#8221; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; max_height=&#8221;90px&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_image][\/et_pb_column][et_pb_column type=&#8221;1_3&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.5&#8243; _module_preset=&#8221;370b430b-b3b2-4e61-8111-d9ef05ce5ad6&#8243; header_4_text_color=&#8221;#C6C6C6&#8243; text_orientation=&#8221;center&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h4>Contact Info<\/h4>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;2023cf4b-5667-411c-ac82-879e66de99ac&#8221; text_text_color=&#8221;#FFFFFF&#8221; link_text_color=&#8221;#FFFFFF&#8221; text_orientation=&#8221;center&#8221; link_option_url=&#8221;https:\/\/maps.app.goo.gl\/CaoyZMXxzzU1jd8o6&#8243; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>2 Shaker Rd., Suite B203<\/p>\n<p>Shirley, MA 01464<\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;2023cf4b-5667-411c-ac82-879e66de99ac&#8221; text_text_color=&#8221;#FFFFFF&#8221; link_text_color=&#8221;#FFFFFF&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;||10px||false|false&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p><a href=\"tel:+15089227719\">+1 (508) 922-7719<\/a><\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;2023cf4b-5667-411c-ac82-879e66de99ac&#8221; text_text_color=&#8221;#FFFFFF&#8221; link_text_color=&#8221;#FFFFFF&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;||10px||false|false&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p><a href=\"mailto:scottedwinlewis4@gmail.com\">scott@legacyantibodies.com<\/a><\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; custom_padding=&#8221;0px||0px||true|&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.20.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_social_media_follow icon_color=&#8221;#000000&#8243; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;||||false|false&#8221; hover_enabled=&#8221;0&#8243; border_radii=&#8221;on|100px|100px|100px|100px&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221; sticky_enabled=&#8221;0&#8243;][et_pb_social_media_follow_network social_network=&#8221;linkedin&#8221; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; background_color=&#8221;#848484&#8243; background_enable_color=&#8221;on&#8221; custom_padding=&#8221;8px|8px|8px|8px|true|true&#8221; global_colors_info=&#8221;{}&#8221; follow_button=&#8221;off&#8221; url_new_window=&#8221;on&#8221; url=&#8221;https:\/\/www.linkedin.com\/company\/legacy-antibodies-llc&#8221; hover_enabled=&#8221;0&#8243; sticky_enabled=&#8221;0&#8243;]linkedin[\/et_pb_social_media_follow_network][\/et_pb_social_media_follow][et_pb_divider color=&#8221;#848484&#8243; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;||0px||false|false&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_divider][et_pb_text _builder_version=&#8221;4.27.5&#8243; _dynamic_attributes=&#8221;content&#8221; _module_preset=&#8221;84a07382-e7a7-4f9c-845a-4ad31f3c7af8&#8243; text_text_color=&#8221;#FFFFFF&#8221; text_orientation=&#8221;center&#8221; locked=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;]@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoiY3VycmVudF9kYXRlIiwic2V0dGluZ3MiOnsiYmVmb3JlIjoiQ29weXJpZ2h0ICZjb3B5OyAiLCJhZnRlciI6IiBMZWdhY3kgQW50aWJvZGllcy4gQWxsIFJpZ2h0cyBSZXNlcnZlZC4iLCJkYXRlX2Zvcm1hdCI6ImN1c3RvbSIsImN1c3RvbV9kYXRlX2Zvcm1hdCI6IlkifX0=@[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>ServicesProteomic AntibodiesAnti-peptide antibodies for use in mass spec assay workflowsAntibodies to tryptic (or other enzyme derived) fragments present an excellent solution for highly accurate and quantifiable measurement of protein targets isolated from biological samples.\u00a0 We at Legacy have been at the forefront of method and protocol development for the creation of these reagents for the [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"<!-- wp:divi\/placeholder \/-->","_et_gb_content_width":"","footnotes":""},"class_list":["post-92","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Service Four - Legacy Antibodies<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/legacyantibodies-m74vhbtrik.live-website.com\/service-four\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Service Four - Legacy Antibodies\" \/>\n<meta property=\"og:description\" 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