{"id":32,"date":"2026-02-22T22:34:33","date_gmt":"2026-02-22T22:34:33","guid":{"rendered":"https:\/\/legacyantibodies.com\/?page_id=32"},"modified":"2026-03-25T18:12:23","modified_gmt":"2026-03-25T18:12:23","slug":"f-a-q","status":"publish","type":"page","link":"https:\/\/legacyantibodies.com\/es\/f-a-q\/","title":{"rendered":"F.A.Q"},"content":{"rendered":"

[et_pb_section fb_built=”1″ admin_label=”Header” _builder_version=”4.27.6″ _module_preset=”default” use_background_color_gradient=”on” background_color_gradient_stops=”rgba(31,35,44,0.7) 0%|gcid-9fa4a1b6-767d-41ca-984c-a6f10449c843 100%” background_color_gradient_overlays_image=”on” background_image=”https:\/\/legacyantibodies.com\/wp-content\/uploads\/2026\/03\/Help-scaled.jpg” custom_padding=”8vw||0vw||false|false” locked=”off” collapsed=”on” global_colors_info=”{%22gcid-9fa4a1b6-767d-41ca-984c-a6f10449c843%22:%91%22background_color_gradient_stops%22%93}”][et_pb_row _builder_version=”4.20.0″ _module_preset=”default” max_width=”800px” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.20.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.6″ _module_preset=”794a691b-ddca-46a8-9f01-65725ba5d48f” header_4_font=”Roboto|700||||on|||” header_4_text_color=”#C6C6C6″ text_orientation=”center” custom_margin=”||10px||false|false” global_colors_info=”{}”]<\/p>\n

FAQs<\/h4>\n

[\/et_pb_text][et_pb_image src=”https:\/\/legacyantibodies.com\/wp-content\/uploads\/2026\/02\/attorney-1.png” title_text=”attorney-1″ _builder_version=”4.20.0″ _module_preset=”default” max_width=”130px” module_alignment=”center” global_colors_info=”{}”][\/et_pb_image][et_pb_text _builder_version=”4.27.6″ _module_preset=”84a1e299-2f39-4535-9b29-4bb9beb77a84″ header_text_color=”#C6C6C6″ text_orientation=”center” global_colors_info=”{}”]<\/p>\n

Legacy Antibodies<\/h1>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.5″ _module_preset=”ac88ca43-8a55-4dbf-83c3-570e1b31c74c” text_text_color=”#C6C6C6″ text_font_size=”17px” header_5_text_color=”#FFFFFF” text_orientation=”center” global_colors_info=”{}”]<\/p>\n

A little help from your friends<\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ admin_label=”Services” _builder_version=”4.20.0″ _module_preset=”default” background_color=”gcid-9fa4a1b6-767d-41ca-984c-a6f10449c843″ min_height=”1002.5px” collapsed=”on” global_colors_info=”{%22gcid-9fa4a1b6-767d-41ca-984c-a6f10449c843%22:%91%22background_color%22%93}”][et_pb_row _builder_version=”4.27.5″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.5″ _module_preset=”default” global_colors_info=”{}”][et_pb_accordion open_toggle_text_color=”#000000″ _builder_version=”4.27.6″ _module_preset=”default” toggle_text_color=”#000000″ body_text_color=”#575959″ background_color=”#1e232b” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_accordion_item title=”What type of antibody is right for me?” open=”off” _builder_version=”4.27.5″ _module_preset=”default” background_color=”#C6C6C6″ background_enable_color=”on” global_colors_info=”{}”]<\/p>\n

This may be simple preference, or depend in part on the desired purpose and\/or overall budget.\u00a0 A helpful chart below lists some generalities about each method, but we are happy to discuss your needs in more detail for the best recommendation.<\/p>\n\n\n\n\n\n\n
\n

Type<\/p>\n<\/td>\n

\n

Cost<\/p>\n<\/td>\n

\n

Time<\/p>\n<\/td>\n

\n

Analysis<\/p>\n<\/td>\n<\/tr>\n

\n

Polyclonal<\/p>\n<\/td>\n

\n

Lowest<\/p>\n<\/td>\n

\n

Lowest<\/p>\n<\/td>\n

\n

Perfect for lab reagents or limited use requirements<\/p>\n

Variation can occur between animals and lots<\/p>\n<\/td>\n<\/tr>\n

\n

Monoclonal<\/p>\n<\/td>\n

\n

Middle<\/p>\n<\/td>\n

\n

Middle<\/p>\n<\/td>\n

\n

No variation<\/p>\n

Finding a \u2018perfect clone\u2019 can be challenging in some situations<\/p>\n

Long term \/ large scale production can be onerous<\/p>\n<\/td>\n<\/tr>\n

\n

Recombinant<\/p>\n<\/td>\n

\n

Highest<\/p>\n<\/td>\n

\n

Highest<\/p>\n<\/td>\n

\n

No variation<\/p>\n

Best for permanent long term \/ large scale needs like drugs or diagnostic reagents<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

[\/et_pb_accordion_item][et_pb_accordion_item title=”How are antibodies made?” _builder_version=”4.27.5″ _module_preset=”default” background_color=”#C6C6C6″ background_enable_color=”on” global_colors_info=”{}” open=”off”]<\/p>\n

The main options available are:<\/p>\n

    \n
  1. Immunizing an animal<\/li>\n
  2. Screening an existing library (aka Phage Display)<\/li>\n
  3. Learning models (AI)<\/li>\n<\/ol>\n

    Animal immunization is the most tried and true method, so this is what we use.\u00a0 Even though the actual response is out of your control, there is a great deal you can do to direct things to optimize the results as best as possible.\u00a0 It might be possible to find a good binder by Phage Display, but because you are selecting from a random library the potential for mixed specificity is higher.\u00a0 There is a lot we can say about AI, but the short version is it just isn\u2019t there yet.<\/p>\n

    [\/et_pb_accordion_item][et_pb_accordion_item title=”What kind of antigen should I use?” _builder_version=”4.27.5″ _module_preset=”default” background_color=”#C6C6C6″ background_enable_color=”on” global_colors_info=”{}” open=”off”]<\/p>\n

    Again, this depends on your need and the particulars at hand.\u00a0 Immunizing with a protein can lead to more \u2018protein specific\u2019 antibodies being made.\u00a0 If your protein is from a larger family with conserved regions or domains, however, the potential for cross-reactivity is higher because you have little control over what part<\/em> of the protein the antibody is made to.\u00a0 Peptides are classically thought of as a risk to generating antibodies that are only \u2018peptide specific\u2019, but making an antibody that reacts with the native protein is also possible when taking factors such as structure (peptide and protein) and surface accessibility into account.<\/p>\n

    [\/et_pb_accordion_item][et_pb_accordion_item title=”How do you know if a conjugation worked?” _builder_version=”4.27.6″ _module_preset=”default” background_color=”#C6C6C6″ background_enable_color=”on” hover_enabled=”0″ global_colors_info=”{}” open=”off” sticky_enabled=”0″]<\/p>\n

    Official data driven QC of a peptide \/ protein conjugate can be difficult to impossible, depending on the protein.\u00a0 Luckily, decades of empirical positive results have shown us that as long as the peptide is in solution prior to conjugation, the selected chemistry should work.\u00a0 Some conjugates may look hazy or even precipitate, but it is still a functional conjugate (albeit at times with reduced usability).\u00a0 Conjugate appearance is typically driven by peptide properties such as hydrophobicity, charge, and pI in solution.<\/p>\n

    [\/et_pb_accordion_item][et_pb_accordion_item title=”Can you provide documentation for approval of animal use?” _builder_version=”4.27.6″ _module_preset=”default” background_color=”#C6C6C6″ background_enable_color=”on” hover_enabled=”0″ global_colors_info=”{}” open=”off” sticky_enabled=”0″]<\/p>\n

    We only contract with approved vendors that operate under official USDA guidelines.\u00a0 Please let us know what you need, and we will be happy to provide what is required.<\/p>\n

    [\/et_pb_accordion_item][et_pb_accordion_item title=”What is your Personal Data Privacy Policy?” _builder_version=”4.27.6″ _module_preset=”default” hover_enabled=”0″ sticky_enabled=”0″ background_color=”#C6C6C6″ background_enable_color=”on” open=”off”]<\/p>\n

    A minimal amount of Personal Data including (but not necessarily limited to): name, contact info, and research interest is collected and kept secure in internal storage.\u00a0 This is primarily used as a means of facilitating free-flowing scientific contact between individuals, but may be also used for internal marketing purposes.\u00a0 This is not shared by Legacy with any outside party, unless required by the services (such as a direct shipment from a 3rd<\/sup> party contractor).\u00a0 Personal Data can be destroyed upon written request.<\/p>\n

    [\/et_pb_accordion_item][et_pb_accordion_item title=”Acceptance Terms and Conditions” _builder_version=”4.27.6″ _module_preset=”default” hover_enabled=”0″ sticky_enabled=”0″ background_color=”#C6C6C6″ background_enable_color=”on” open=”on”]<\/p>\n

    By supplying payment information for an Order the person named therein (the \u201cCustomer<\/u>\u201d) agrees to be bound by and comply with certain terms and conditions of purchase (hereinafter, the \u201cTerms and Conditions<\/u>\u201d), including any properly authorized supplements thereto which are incorporated herein by reference, and are collectively referred to as the \u201cAgreement<\/u>.\u201d\u00a0 These set forth the entire Terms and Conditions applicable to the manufacture and supply by Legacy Antibodies LLC, a Massachusetts limited liability company (\u201cLegacy<\/u>\u201d), of goods and services (the \u201cMaterials<\/u>\u201d) for sale to Customer.\u00a0 All sales are subject to and expressly conditioned upon these Terms and Conditions and upon Customer\u2019s consent thereto.<\/p>\n

      \n
    1. Use of Materials.<\/strong> Legacy supplies Materials solely for research use by Customer.\u00a0 Customer understands that the Materials are not made to GMP standards and are not intended for use in humans.<\/li>\n
    2. Changes and Cancellation.<\/strong> Legacy reserves the right to refuse or cancel any Order in whole or in part by notice to Customer if Legacy reasonably determines that it cannot successfully or safely produce the Materials as ordered, or that the manufacture, sale or use of the ordered Materials is likely to violate any applicable law or regulation.\u00a0 This Order may not be changed or cancelled by Customer unless such change or cancellation is expressly agreed to in writing by Legacy.<\/li>\n
    3. Reservation of Rights. <\/strong>Legacy acknowledges that any detailed specifications for any custom product (\u201cthe Specifications\u201d) shall remain Customer\u2019s property.\u00a0 The parties agree that Legacy may electronically document its scientific observations and results therefrom relating to Legacy\u2019s performance under this Agreement, and Legacy may use such data to generally provide Materials for its customers.\u00a0 Nothing contained in this Agreement shall grant or transfer to Customer any right, title or interest in any of Legacy\u2019s patents, copyrights, trademarks, trade secrets or other proprietary rights.<\/li>\n
    4. Payment and Delivery.<\/strong> All prices are FOB a facility of Legacy\u2019s designation and are exclusive of further costs, including, but not limited to, shipping and handling and all domestic and foreign taxes, excises, duties and the like, which further costs Customer shall be responsible for paying in addition to the quoted prices. Payment terms are net thirty (30) days from the date of Material shipment to Customer, unless otherwise stipulated in a separate agreement.\u00a0 Legacy reserves the right to ship and invoice Customer for partial orders if one or more units is delayed for any reason.\u00a0 In the event of a payment default, Customer shall be responsible for all of Legacy\u2019s costs of collection, including court costs, filing fees and attorney\u2019s fees.<\/li>\n
    5. Legacy<\/strong> Representations and Warranties.<\/strong> The Materials are provided to Customer \u201cas-is\u201d and without indemnity, warranty, or guarantee, whether express or implied, and whether of merchantability, fitness for a particular purpose, non-infringement, title, or otherwise.\u00a0 The Customer only shall be liable for any use if the Materials or the progeny or derivatives, and for any recall, loss, claim, damage, or liability of any kind or nature, which may arise from or in connection with the use, handling or storage of the Materials or the progeny or derivatives after delivery to Customer.<\/li>\n
    6. Customer Representations and Warranties.<\/strong> Customer\u2019s submission of the Order conclusively constitutes Customer\u2019s representation and warranty that neither the Specifications nor Legacy\u2019s or the Customer\u2019s use of them or the Materials will violate any applicable law or regulation, or misappropriate or infringe upon any intellectual property and other proprietary rights, contracts or other third party interests anywhere in the world, including without limitation laws as to hazardous or infectious materials or agents, the use or release of modified organisms, patents, copyrights, trademarks, trade names, know-how protection, trade secrets and exports.<\/li>\n
    7. Indemnification.<\/strong> Customer agrees to indemnify Legacy and its affiliates and their officers, agents and employees from and against any liability or loss incurred by any of them to the extent arising out of the Specifications or out of Customer\u2019s, or any of Customer\u2019s affiliates, use of the Materials or their progeny or derivatives.<\/li>\n
    8. Special Damages.<\/strong> Legacy shall in no event be liable to Customer or any third party for any indirect, incidental, consequential, punitive or special damages arising out of this Agreement or any use of the Materials.\u00a0 In no event shall Legacy\u2019s aggregate liability under or in relation to this Agreement or the sale of the Materials exceed the amount paid by Customer to Legacy for the purchase price of the Materials under this Agreement.<\/li>\n
    9. Termination.<\/strong> Either party may terminate this Agreement at any time by giving the other party at least thirty (30) days\u2019 advance notice.<\/li>\n
    10. Miscellaneous.<\/strong> Legacy\u2019s failure to strictly enforce any of these Terms and Conditions or to exercise any right arising under this Agreement shall not constitute a waiver of Legacy\u2019s right to strictly enforce such Terms and Conditions or exercise such right thereafter. All rights and remedies under this Order are cumulative and are in addition to any other rights and remedies Legacy may have at law or in equity.\u00a0 Any waiver by Legacy of a default by Customer hereunder shall be in writing and shall not operate as a waiver of any other default or of the same default thereafter.\u00a0 This Agreement shall be binding upon, inure to the benefit of, and be enforceable by, the parties hereto, and their respective affiliates, heirs, personal representatives, successors and assigns. \u00a0No variation of these Terms and Conditions shall be binding upon Legacy.\u00a0 If any provision of these Terms and Conditions is for any reason found illegal, invalid or unenforceable under present or future laws, such provision shall be fully severable and such finding or holding shall not affect the legality, validity or enforceability of the remaining provisions of these Terms and Conditions.\u00a0 If Legacy is in whole or in part unable to perform any of its obligations under this Agreement due to causes beyond its reasonable control, such performance shall be excused or the time for such performance shall be extended.\u00a0 To the extent that any of these Terms and Conditions conflict with any term, condition, or provision contained in another agreement between the parties, then the applicable term, condition, or provision contained in any of the following agreements will prevail in priority order as follows, as applicable: (1) Manufacturing and Supply Agreement, (2) Confidentiality Agreement, (3) these Terms and Conditions.\u00a0 Any notice to either party required or permitted under this Agreement shall be in writing and shall be effective upon receipt at such party\u2019s regular business address.\u00a0 This Agreement and all related acts and transactions shall be governed, construed and interpreted in accordance with the laws of the Commonwealth of Massachusetts, USA.<\/li>\n<\/ol>\n

      [\/et_pb_accordion_item][\/et_pb_accordion][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ admin_label=”Footer” _builder_version=”4.27.6″ _module_preset=”default” use_background_color_gradient=”on” background_color_gradient_stops=”#1e232b 0%|rgba(30,35,43,0.9) 100%” background_color_gradient_overlays_image=”on” background_enable_image=”off” saved_tabs=”all” locked=”off” collapsed=”on” global_colors_info=”{}”][et_pb_row column_structure=”1_3,1_3,1_3″ _builder_version=”4.20.0″ _module_preset=”default” locked=”off” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.20.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.5″ _module_preset=”370b430b-b3b2-4e61-8111-d9ef05ce5ad6″ header_4_text_color=”#C6C6C6″ text_orientation=”center” global_colors_info=”{}”]<\/p>\n

      Office Hours<\/h4>\n

      [\/et_pb_text][et_pb_text _builder_version=”4.27.5″ _module_preset=”2023cf4b-5667-411c-ac82-879e66de99ac” text_text_color=”#FFFFFF” text_orientation=”center” custom_margin=”||10px||false|false” global_colors_info=”{}”]<\/p>\n

      M – F 7:00 am to 4:00 pm (ET)<\/p>\n

      [\/et_pb_text][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”4.20.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/legacyantibodies.com\/wp-content\/uploads\/2026\/03\/Logo-final.png” title_text=”Logo final” align=”center” _builder_version=”4.27.6″ _module_preset=”default” background_enable_color=”off” max_height=”90px” global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”4.20.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.5″ _module_preset=”370b430b-b3b2-4e61-8111-d9ef05ce5ad6″ header_4_text_color=”#C6C6C6″ text_orientation=”center” locked=”off” global_colors_info=”{}”]<\/p>\n

      Contact Info<\/h4>\n

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      2 Shaker Rd., Suite B203<\/p>\n

      Shirley, MA 01464<\/p>\n

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      +1 (508) 922-7719<\/a><\/p>\n

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      scott@legacyantibodies.com<\/p>\n

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      FAQsLegacy AntibodiesA little help from your friendsThis may be simple preference, or depend in part on the desired purpose and\/or overall budget.\u00a0 A helpful chart below lists some generalities about each method, but we are happy to discuss your needs in more detail for the best recommendation. Type Cost Time Analysis Polyclonal Lowest Lowest Perfect […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-32","page","type-page","status-publish","hentry"],"yoast_head":"\nF.A.Q - Legacy Antibodies<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/legacyantibodies.com\/es\/f-a-q\/\" \/>\n<meta property=\"og:locale\" content=\"es_MX\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"F.A.Q - Legacy Antibodies\" \/>\n<meta property=\"og:description\" content=\"FAQsLegacy AntibodiesA little help from your friendsThis may be simple preference, or depend in part on the desired purpose and\/or overall budget.\u00a0 A helpful chart below lists some generalities about each method, but we are happy to discuss your needs in more detail for the best recommendation. 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