Legacy Antibodies

The main options available are:

1. Immunizing an animal
2. Screening an existing library (aka Phage Display)
3. Learning models (AI)

Animal immunization is the most tried and true method, so this is what we use.  Even though the actual response is out of your control, there is a great deal you can do to direct things to optimize the results as best as possible.  It might be possible to find a good binder by Phage Display, but because you are selecting from a random library the potential for mixed specificity is higher.  There is a lot we can say about AI, but the short version is it just isn’t there yet.

Again, this depends on your need and the particulars at hand.  Immunizing with a protein can lead to more ‘protein specific’ antibodies being made.  If your protein is from a larger family with conserved regions or domains, however, the potential for cross-reactivity is higher because you have little control over what part of the protein the antibody is made to.  Peptides are classically thought of as a risk to generating antibodies that are only ‘peptide specific’, but making an antibody that reacts with the native protein is also possible when taking factors such as structure (peptide and protein) and surface accessibility into account.

Official data driven QC of a peptide / protein conjugate can be difficult to impossible, depending on the protein.  Luckily, decades of empirical positive results have shown us that as long as the peptide is in solution prior to conjugation, the selected chemistry should work.  Some conjugates may look hazy or even precipitate, but it is still a functional conjugate (albeit at times with reduced usability).  Conjugate appearance is typically driven by peptide properties such as hydrophobicity, charge, and pI in solution.

We only contract with approved vendors that operate under official USDA guidelines.  Please let us know what you need, and we will be happy to provide what is required.

A minimal amount of Personal Data including (but not necessarily limited to): name, contact info, and research interest is collected and kept secure in internal storage.  This is primarily used as a means of facilitating free-flowing scientific contact between individuals, but may be also used for internal marketing purposes.  This is not shared by Legacy with any outside party, unless required by the services (such as a direct shipment from a 3^rd party contractor).  Personal Data can be destroyed upon written request.

By supplying payment information for an Order the person named therein (the “Customer”) agrees to be bound by and comply with certain terms and conditions of purchase (hereinafter, the “Terms and Conditions”), including any properly authorized supplements thereto which are incorporated herein by reference, and are collectively referred to as the “Agreement.”  These set forth the entire Terms and Conditions applicable to the manufacture and supply by Legacy Antibodies LLC, a Massachusetts limited liability company (“Legacy”), of goods and services (the “Materials”) for sale to Customer.  All sales are subject to and expressly conditioned upon these Terms and Conditions and upon Customer’s consent thereto.

1. Use of Materials. Legacy supplies Materials solely for research use by Customer.  Customer understands that the Materials are not made to GMP standards and are not intended for use in humans.
2. Changes and Cancellation. Legacy reserves the right to refuse or cancel any Order in whole or in part by notice to Customer if Legacy reasonably determines that it cannot successfully or safely produce the Materials as ordered, or that the manufacture, sale or use of the ordered Materials is likely to violate any applicable law or regulation.  This Order may not be changed or cancelled by Customer unless such change or cancellation is expressly agreed to in writing by Legacy.
3. Reservation of Rights. Legacy acknowledges that any detailed specifications for any custom product (“the Specifications”) shall remain Customer’s property.  The parties agree that Legacy may electronically document its scientific observations and results therefrom relating to Legacy’s performance under this Agreement, and Legacy may use such data to generally provide Materials for its customers.  Nothing contained in this Agreement shall grant or transfer to Customer any right, title or interest in any of Legacy’s patents, copyrights, trademarks, trade secrets or other proprietary rights.
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5. Legacy Representations and Warranties. The Materials are provided to Customer “as-is” and without indemnity, warranty, or guarantee, whether express or implied, and whether of merchantability, fitness for a particular purpose, non-infringement, title, or otherwise.  The Customer only shall be liable for any use if the Materials or the progeny or derivatives, and for any recall, loss, claim, damage, or liability of any kind or nature, which may arise from or in connection with the use, handling or storage of the Materials or the progeny or derivatives after delivery to Customer.
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